The following table depicts the main nutritional composition of Hydrolysate Beef protein and Isolate whey protein powder.
|Nutrient||100% All Beef||Whey Isolac|
|20 g||100 g||20 g||100 g|
|Energy value (kcal)||82||410||78||390|
Saturated Fatty Acid (g)
Polyunsaturated fatty Acid (g)
Mon saturated Fatty Acid (g)
|Amino acid Profile|
|Total Essential Amino Acid||6.82||34.1||8.91||44.55|
|Heme Iron (mg)||3.4||17.0||–|
|Folic Acid (µg)||16.94||84.71||~|
|Vitamin B 12 (µg)||0.68||3.41||~|
|Vitamin B6 (mg)||0.064||0.32||~|
|Pantotenic Acid B5 (mg)||0.23||1.14||–|
|Vitamin D (µg)||0.25||1.26||~|
Both protein supplements seems to be particularly rich in EAA including Leucine; which acts as a key amino acid to stimulate the muscle protein synthesis (Dideriksen et al., 2013). It has been estimated that about 0.25g·kg-1 (Witard et al., 2014) up to ~0.40 g·kg-1 (Macnaughton et al., 2016) of high-quality protein providing between ~90 to ~230 mg·kg-1 of essential amino acid (EAA) with 2 to 3 g of leucine (20 to 30 mg·kg-1) consumed after exercise may maximize rates of muscle protein synthesis in young individuals. E.g. for a 70 kg man consuming a typical dose of 20 g of the powder product presented in the table, about 0.23 or 0.26 g·kg-1 of protein would be ingested from 100% All Beef and Whey Isolac respectively. This amount falls within the 90% confidence interval (0.18 to 0.30 g·kg-1) of effective protein dose to promote muscle protein synthesis after exercise (Morton et al., 2015).
Whey isolate provides higher amount of EAA (8.91 or 127 mg·kg-1 vs. 6.82 or 97 mg·kg-1 for a 70kg person) and leucine (1.93 vs. 1.32), respectively. However, despite the aforementioned differences the amount of EAA, including leucine, provided by a 20 g dose of 100% All Beef for a 70kg person would reach a threshold to effectively support muscle protein synthesis and training adaptations in a similar way as Whey Isolac does (Naclerio et al., 2017a, Naclerio and Larumbe-Zabala, 2016). Furthermore other nutritional aspect as the micronutrient composition of the protein sources (e.g. iron, zinc, vitamin B12 or essential fatty acid included in beef) would also represent relevant factors for supporting training outcomes (Phillips, 2012, Naclerio et al, 2017b).
DIDERIKSEN, K., REITELSEDER, S. & HOLM, L. 2013. Influence of amino acids, dietary protein, and physical activity on muscle mass development in humans. Nutrients, 5, 852-76.
MACNAUGHTON, L. S., WARDLE, S. L., WITARD, O. C., MCGLORY, C., HAMILTON, D. L., JEROMSON, S., LAWRENCE, C. E., WALLIS, G. A. & TIPTON, K. D. 2016. The response of muscle protein synthesis following whole-body resistance exercise is greater following 40 g than 20 g of ingested whey protein. Physiol Rep, 4.
MORTON, R. W., MCGLORY, C. & PHILLIPS, S. M. 2015. Nutritional interventions to augment resistance training-induced skeletal muscle hypertrophy. Front Physiol, 6, 245.
NACLERIO, F. & LARUMBE-ZABALA, E. 2016. Effects of Whey Protein Alone or as Part of a Multi-ingredient Formulation on Strength, Fat-Free Mass, or Lean Body Mass in Resistance-Trained Individuals: A Meta-analysis. Sports Med, 46, 125-37.
NACLERIO, F., LARUMBE-ZABALA, E., ASHRAFI, N., SEIJO, M., NIELSEN, B., ALLGROVE, J. & EARNEST, C. P. 2017. Effects of protein-carbohydrate supplementation on immunity and resistance training outcomes: a double-blind, randomized, controlled clinical trial. Eur J Appl Physiol, 117, 267-277.
NACLERIO, F., SEIJO, M., LARUMBE-ZABALA, E., & EARNEST, C. P.Carbohydrates Alone or Mixing With Beef or Whey Protein Promote Similar Training Outcomes in Resistance Training Males: A Double Blind, Randomized Controlled Clinical Trial. Int J Sport Nutr Exerc Metab, in press 2017
PHILLIPS, S. M. 2012. Nutrient-rich meat proteins in offsetting age-related muscle loss. Meat Sci, 92, 174-8.
WITARD, O. C., JACKMAN, S. R., BREEN, L., SMITH, K., SELBY, A. & TIPTON, K. D. 2014. Myofibrillar muscle protein synthesis rates subsequent to a meal in response to increasing doses of whey protein at rest and after resistance exercise. Am J Clin Nutr, 99, 86-95.